Important Events And Discoveries In Biotechnology

1977:

The Age of biotechnology arrives with “somatostatin” – a human growth hormone-releasing inhibitory factor, the first human protein manufactured in bacteria by Genentech, Inc. A synthetic, recombinant gene was used to clone a protein for the first time.

1978:

Genentech, Inc. and The City of Hope National Medical Center announce the successful laboratory production of human insulin using recombinant DNA technology. Hutchinson and Edgell show it is possible to introduce specific mutations at specific sites in a DNA molecule.

1979:

Sir Walter Bodmer suggests a way of using DNA technology to find gene markers to show up specific genetic diseases and their carriers. John Baxter reports cloning the gene for human growth hormone.

1980:

The prokaryote model, E. coli, is used to produce insulin and other medicine, in human form. Researchers successfully introduce a human gene – one that codes for the protein interferon- into a bacterium. The U.S. patent for gene cloning is awarded to Cohen and Boyer.

1981:

Scientists at Ohio University produce the first transgenic animals by transferring genes from other animals into mice. The first gene-synthesizing machines are developed. Chinese scientists successfully clone a golden carp fish.

1982:

Genentech, Inc. receives approval from the Food and Drug Administration to market genetically engineered human insulin. Applied Biosystems, Inc. introduces the first commercial gas phase protein sequencer.

1983:

The polymerase chain reaction is invented by Kary B Mullis. The first artificial chromosome is synthesized, and the first genetic markers for specific inherited diseases are found.

1984:

Chiron Corp. announces the first cloning and sequencing of the entire human immunodeficiency virus (HIV) genome. Alec Jeffreys introduces technique for DNA fingerprinting to identify individuals. The first genetically engineered vaccine is developed.

1985:

Cetus Corporation’s develops GeneAmp polymerase chain reaction (PCR) technology, which could generate billions of copies of a targeted gene sequence in only hours. Scientists find a gene marker for cystic fibrosis on chromosome number 7.

1986:

The first genetically engineered human vaccine – Chiron’s Recombivax HB – is approved for the prevention of hepatitis B. A regiment of scientists and technicians at Caltech and Applied Biosystems, Inc. invented the automated DNA fluorescence sequencer.

1987:

The first outdoor tests on a genetically engineered bacterium are allowed. It inhibits frost formation on plants. Genentech’s tissue plasminogen activator (tPA), sold as Activase, is approved as a treatment for heart attacks.

1988:

Harvard molecular geneticists Philip Leder and Timothy Stewart awarded the first patent for a genetically altered animal, a mouse that is highly susceptible to breast cancer

1989:

UC Davis scientists develop a recombinant vaccine against the deadly rinderpest virus. The human genome project is set up, a collaboration between scientists from countries around the world to work out the whole of the human genetic code.

1990:

The first gene therapy takes place, on a four-year-old girl with an immune-system disorder called ADA deficiency. The human genome project is formally launched.

1991:

Mary-Claire King, of the University of California, Berkeley, finds evidence that a gene on chromosome 17 causes the inherited form of breast cancer and also increases the risk of ovarian cancer. Tracey the first transgenic sheep is born.

1992:

The first liver xenotransplant from one type of animal to another is carried out successfully. Chiron’s Proleukin is approved for the treatment of renal cell cancer.

1993:

The FDA declares that genetically engineered foods are “not inherently dangerous” and do not require special regulation. Chiron’s Betaseron is approved as the first treatment for multiple sclerosis in 20 years.

1994:

The first genetically engineered food product, the Flavr Savr tomato, gained FDA approval. The first breast cancer gene is discovered. Genentech’s Nutropin is approved for the treatment of growth hormone deficiency.

1995:

Researchers at Duke University Medical Center transplanted hearts from genetically altered pigs into baboons, proving that cross-species operations are possible. The bacterium Haemophilus influenzae is the first living organism in the world to have its entire genome sequenced.

1996:

Biogen’s Avonex is approved for the treatment of multiple sclerosis. The discovery of a gene associated with Parkinson’s disease provides an important new avenue of research into the cause and potential treatment of the debilitating neurological ailment.

1997:

Researchers at Scotland’s Roslin Institute report that they have cloned a sheep–named Dolly–from the cell of an adult ewe. The FDA approves Rituxan, the first antibody-based therapy for cancer.

1998:

The first complete animal genome the C.elegans worm is sequenced. James Thomson at Wisconsin and John Gearhart in Baltimore each develop a technique for culturing embryonic stem cells.

1999:

A new medical diagnostic test will for the first time allow quick identification of BSE/CJD a rare but devastating form of neurologic disease transmitted from cattle to humans.

2000:

“Golden Rice,” modified to make vitamin A. Cloned pigs are born for the first time in work done by Alan Coleman and his team at PPL, the Edinburgh-based company responsible for Dolly the sheep.

2001:

The sequence of the human genome is published in Science and Nature, making it possible for researchers all over the world to begin developing genetically based treatments for disease.

2002:

Researchers sequence the DNA of rice, and is the first crop to have its genome decoded.

2003:

The sequencing of the human genome is completed.

Big Bang Booming – Back To The Future

Theoretical cosmologists spend much of their time perfecting what is now known as the ‘Big Bang’ theory. This concept originates from ideas percolating in the minds of scientists, theologians and astronomers down through the ages. However, much of what they consider as proof for the ‘Big Bang’ is dependent upon uncontrolled experimentation that is molded to meet their expectations.

Then God said, “Let there be light,” and there was light. This ancient description of the creation of the universe found in the Book of Genesis may be accurate after all. The big bang theory describes the beginning of the universe as having been precipitated from an infinitesimally small point. In this small volume, all matter and energy was concentrated until its contents exploded in either a smooth expansion or an incredibly violent energetic explosion that formed the planets, stars and galaxies. Originally this theory had competition from what is called the ’steady state’ theory whereby the universe is forever expanding and new matter and energy is created spontaneously within the space left by the receding galaxies. However, empirical observations have directed astronomers and scientists into the acceptance of the big bang model. But how did we get to this point in our understanding?

In the early part of the twentieth century the American astronomer Vesto Slipher and the German Carl Wirtz made some important astronomical discoveries. Using spectral analysis, Slipher deciphered the mixtures of gases contained in planetary atmospheres as well as nebulae. What distinguishes his findings is the discovery that most if not all galaxies outside of our own demonstrate what is called a ‘Red Shift.’ This shift is simply a change in the wavelength of the light emitted by those objects under investigation towards a longer wavelength. Wirtz similarly catalogued many red shifts of the nebulae which he chose to study. But it was still to early for them to realize the full potential meaning of their observations. That would wait until Einstein’s General Relativity would be interpreted by other scientists through further mathematical analysis.

His contemporaries demonstrated to Einstein that his new Theory of General Relativity published in 1916 was not compatible with a ’static’ universe of space time. The theory predicted an expanding or collapsing universe but not a fixed cosmos. Because he personally believed the universe to be an invariable space time continuum, Einstein engaged in a degree of scientific legerdemain. To correct what he perceived to be as ‘flaws’ in his theory he added the contrivance of a cosmological constant known as lambda to force the static universe into reality. Einstein’s view of perfection in an unchanging space time continuum had led him down a blind alley as much as Aristotle’s concept of perfection had brought that great philosopher into the error of believing in a static Earth at the center of the universe.

But even with the addition of the cosmological constant lambda, the universe was still found to be unstable and this whole affair would later be viewed by Einstein as his “greatest blunder.” His cosmological acrobatics behind him, Einstein yielded the stage to others for a clearer understanding of his own theory. It fell to Alexander Alexandrovich Friedmann to consider the consequences of General Relativity without the constant lambda interfering with his study of these relationships. In doing so, the Russian mathematician and cosmologist derived the solution which predicts an ever expanding cosmological structure (1922), a prediction which was disagreeable with Einstein’s concept of universal perfection. A couple of years later, Friedmann published his findings in “About the Possibility of a World with Constant Negative Curvature of Space.” But the entire hypothetical construct still lacked a complete verbalization mathematically and theoretically.

Enter the Reverend Father Georges Lemaitre, a Catholic priest from Belgium. Rev. Fr. Lemaitre provided the equations necessary to formulate the basis of Big Bang theory in his work entitled “Hypothesis of the Primeval Atom.” He postulated that the universe began as a primordial atom of infinitesimal volume and enormous mass energy as well as space and time and everything else comprising the future universe. At some point the universe began with the explosion of this super atom. Lemaitre published his theoretical ideas between the years 1927 and 1933 and speculated that the movement of the nebulae demonstrated the validity of the explosion of his cosmic super atom. Unfortunately, he also wrongly believed that cosmic rays might be an after effect of the super atom’s big bang. These are now known to be generated not from a universal conflagration but from galactic sources unrelated to the big bang.

However, the new theory still lacked a major source of observational support. This would be provided by Edwin Hubble’s observations of the redshift of galaxies. Taking up where Slipher and Wirtz left off, Hubble employed a novel technique to discern the properties of the galactic movements. By choosing to observe stars that are known as Cepheid Variables he could more accurately make measurements. Cepheids are a type of star that brighten and darken and lighten back up in regular periods of time that are well known. Cepheids that have identical cycle times of brightening darkening and brightening again also have identical or nearly identical luminosity. Thus, if one compares the length of the cycle to the amount of light apparent to the observer it is possible to accurately prepare an estimate of the distance to the cepheid.

In this manner, Hubble had found that the nebulae or galaxies exhibited a galactic red shift; in other words, that galaxies were receding away from ours at a speed which is correlated directly with the distance between our vantage point and the galaxy being studied. The further away the galaxies were the faster they appeared to be going in moving away from us. The results of these investigations is now known as Hubble’s Law. Essentially, this law states that universe is in an ever expanding mode whereby the intergalactic distances continue to grow without bound into infinity. Hubble’s Law depends upon the shifting of the wavelength of light and after having been delineated in 1929 has been subsequently proven over and over again. Further, Hubble’s constant has been recalculated to a more ‘perfect’ value and retains a great probability of being ‘recomputed’ in the future based upon new observations.

Thus, it should be clear to the reader that our scientists have a fateful habit of introducing their preconceived notions of beauty into their models. From Aristotle’s static Earth to Einstein’s greatest blunder, the constant which forces a static universe, we proceed only from the wisdom of our weak minds. The more things change the more things stay the same. Man’s hubris knows no limits in our attempts to understand things without the wisdom to comprehend its underlying meaning. Humble we are not. We are making the same mistakes we always have.